Premium
An integrated microfluidic device in polyester for electrophoretic analysis of amino acids
Author(s) -
Guo Yanli,
Uchiyama Katsumi,
Nakagama Tatsuro,
Shimosaka Takuya,
Hobo Toshiyuki
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410126
Subject(s) - chromatography , microfluidics , chemistry , glycine , microchannel , phenylalanine , amino acid , detection limit , fluorescence , analytical chemistry (journal) , electrophoresis , materials science , nanotechnology , optics , biochemistry , physics
A precolumn reaction chamber was integrated into a polyester microfluidic device with a miniaturized detection system. The reaction chamber was designed to be a zigzag channel, 70 μm in width, 8 mm in length, followed by a wider straight channel, 150 μm in width, 2 mm in length. The detection system is composed of an embedded light‐emitting diode (LED), an integrated optical fiber, and a photomultiplier tube (PMT). A success in amino acid analysis using the integrated microchemical analysis device proved that the precolumn reaction chamber was compatible with the integrated detection system. Three kinds of amino acids, arginine, glycine, and phenylalanine, mixed and reacted with 7‐fluoro‐4‐nitrobenzo‐2‐oxa‐1,3‐diazole (NBD‐F) in the precolumn reaction chamber to produce fluorescent products, were separated by micellar eletrokinetic chromatography (MEKC) and detected by LED‐excited fluorescence. The detection limits for arginine, glycine, and phenylalanine were 1, 1, and 0.5 m M , respectively, which can be improved by further optimizations of the reaction system and detection system.