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Speciation of arsenic compounds in fish and oyster tissues by capillary electrophoresis‐inductively coupled plasma‐mass spectrometry
Author(s) -
Yeh ChingFen,
Jiang ShiuhJen
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410116
Subject(s) - inductively coupled plasma mass spectrometry , capillary electrophoresis , chemistry , genetic algorithm , mass spectrometry , arsenic , oyster , fish <actinopterygii> , chromatography , environmental chemistry , analytical chemistry (journal) , fishery , biology , organic chemistry , evolutionary biology
A capillary electrophoresis‐inductively coupled plasma‐mass spectrometric (CE‐ICP‐MS) method for the speciation of six arsenic compounds, namely arsenite [As(III)], arsenate [As(V)], monomethylarsonic acid, dimethylarsinic acid, arsenobetaine and arsenocholine is described. The separation has been achieved on a 70 cm length×75 µm ID fused‐silica capillary. The electrophoretic buffer used was 15 m M Tris (pH 9.0) containing 15 m M sodium dodecyl sulfate (SDS), while the applied voltage was set at +22 kV. The arsenic species in biological tissues were extracted into 80% v/v methanol‐water mixture, put in a closed centrifuge tube and kept in a water bath, using microwaves at 80°C for 3 min. The extraction efficiencies of individual arsenic species added to the sample at 0.5 µg As/g level were between 96% and 107%, except for As(III), for which it was 89% and 77% for oyster and fish samples, respectively. The detection limits of the species studied were in the range 0.3–0.5 ng As/mL. The procedure has been applied for the speciation analysis of two reference materials, namely dogfish muscle tissue (NRCC DORM‐2) and oyster tissue (NIST SRM 1566a), and two real‐world samples.