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Capillary electrophoresis direct enantioseparation of aromatic amino acids based on mixed chelate‐inclusion complexation of aminoethylamino‐β‐cyclodextrin
Author(s) -
Wu Bidong,
Wang Qingqing,
Liu Qin,
Xie Jianwei,
Yun Liuhong
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200410078
Subject(s) - capillary electrophoresis , cyclodextrin , chemistry , chelation , inclusion (mineral) , chromatography , organic chemistry , mineralogy
6 A ‐(2‐Aminoethylamino)‐6 A ‐deoxy‐β‐cyclodextrin (CDen) was synthesized and formed a binary complex with Cu(II) which was shown to be an effective chiral selector for separation of underivatized amino acid enantiomers in capillary electrophoresis (CE). Moreover, the chiral resolution was greatly enhanced by the presence of polyethyl glycol (PEG) and tert ‐butyl alcohol in the running buffer. The optimum experimental conditions were 20 mmol/L CDen, 20 mmol/L CuSO 4 ·5H 2 O, 5.0 mg/mL PEG20000 and 1.0% v/v tert ‐butyl alcohol, pH 5.80. With the proposed method, the four selected aromatic chiral amino acid pairs were separated in less than 15 min.