Premium
Separation of perfluorocarboxylic acids using capillary electrophoresis with UV detection
Author(s) -
Wójcik Lena,
Szostek Bogdan,
Maruszak Wioleta,
Trojanowicz Marek
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406184
Subject(s) - chromatography , capillary electrophoresis , chemistry , detection limit , stacking , electrolyte , electrophoresis , chromophore , analytical chemistry (journal) , electrode , organic chemistry
A capillary electrophoretic method with UV detection for separation and quantitation of perfluorocarboxylic acids (PFCAs) from C6‐PFCA to C12‐PFCA has been developed. The optimization of measurement conditions included the choice of the most appropriate type and concentration of buffer in the background electrolyte (BGE), as well as the type and the content of an organic modifier. The optimal separation of investigated PFCAs was achieved with 50 m M phosphate buffer and 40% isopropanol in the BGE using direct UV detection. The optimum wavelength for direct UV detection was optimized at 190 nm. For indirect detection, several chromophores were studied. Five m M 3,5‐Dinitrobenzoic acid (3,5‐DNBA) in 20 m M phosphate buffer BGE and indirect UV detection at 280 nm gave the optimal detection and separation performance for the investigated PFCAs. The possibility of on‐line preconcentration of solutes by stacking has been examined for indirect detection. The detection limits (LODs) determined for direct UV detection ranged from 2 µg/mL for C6‐PFCA to 33 µg/mL for C12‐PFCA. The LODs obtained for indirect UV detection were comparable to those obtained for direct UV detection.