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Multilayer poly(vinyl alcohol)‐adsorbed coating on poly(dimethylsiloxane) microfluidic chips for biopolymer separation
Author(s) -
Wu Dapeng,
Luo Yong,
Zhou Xiaomian,
Dai Zhongpeng,
Lin Bingcheng
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406157
Subject(s) - vinyl alcohol , biopolymer , adsorption , coating , bovine serum albumin , chemical engineering , chemistry , lysozyme , protein adsorption , microfluidics , hydrolysis , polymer , layer (electronics) , materials science , chromatography , polymer chemistry , organic chemistry , nanotechnology , biochemistry , engineering
A poly(dimethylsiloxane) (PDMS) microfluidic chip surface was modified by multilayer‐adsorbed and heat‐immobilized poly(vinyl alcohol) (PVA) after oxygen plasma treatment. The reflection absorption infrared spectrum (RAIRS) showed that 88% hydrolyzed PVA adsorbed more strongly than 100% hydrolyzed one on the oxygen plasma‐pretreated PDMS surface, and they all had little adsorption on original PDMS surface. Repeating the coating procedure three times was found to produce the most robust and effective coating. PVA coating converted the original PDMS surface from a hydrophobic one into a hydrophilic surface, and suppressed electroosmotic flow (EOF) in the range of pH 3–11. More than 1 000 000 plates/m and baseline resolution were obtained for separation of fluorescently labeled basic proteins (lysozyme, ribonuclease B). Fluorescently labeled acidic proteins (bovine serum albumin, β‐lactoglobulin) and fragments of dsDNA ϕX174 RF/ Hae III were also separated satisfactorily in the three‐layer 88% PVA‐coated PDMS microchip. Good separation of basic proteins was obtained for about 70 consecutive runs.