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Parallel isoelectric focusing II
Author(s) -
Zilberstein Gleb V.,
Baskin Emmanuil M.,
Bukshpan Shmuel,
Korol Leonid E.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406117
Subject(s) - isoelectric focusing , miniaturization , isoelectric point , buffer (optical fiber) , electrophoresis , resolution (logic) , chromatography , pid controller , sensitivity (control systems) , chemistry , computer science , analytical chemistry (journal) , materials science , nanotechnology , electronic engineering , temperature control , engineering , artificial intelligence , control engineering , telecommunications , biochemistry , enzyme
A miniature electrophoretic device is developed on the basis of a new isoelectric focusing (IEF) method, namely parallel isoelectric focusing. We report here the theory and the results of operation of a new parallel isoelectric device (PID). The main advantages and limitations of the method are discussed for miniaturization purposes. It is shown that the method guarantees the fast and complete separation of any complex protein mixtures under acceptable conditions, such as voltage source, temperature, size of the device, and separation process duration. It is shown that the main problem of PID miniaturization is the buffer design, and the relation between Immobiline buffer capacity and solution buffer capacity. The main experimental limitation of PID resolution is protein sensitivity to pH changes.