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New directions of miniaturization within the biomarker research area
Author(s) -
MarkoVarga György A.,
Nilsson Johan,
Laurell Thomas
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406109
Subject(s) - miniaturization , proteome , proteomics , protein chip , mass spectrometry , protein expression , nanotechnology , profiling (computer programming) , protein detection , computational biology , biomarker , chromatography , computer science , chemistry , bioinformatics , biology , materials science , biochemistry , gene , operating system
An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel and liquid phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low‐abundant protein expressions where assay miniaturization and integrated sample processing play a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.