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Autonomous protein sample processing on‐chip using solid‐phase microextraction, capillary force pumping, and microdispensing
Author(s) -
Wallman Lars,
Ekström Simon,
MarkoVarga György,
Laurell Thomas,
Nilsson Johan
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406100
Subject(s) - chromatography , chemistry , elution , solid phase microextraction , mass spectrometry , capillary action , analytical chemistry (journal) , sample preparation , desorption , microsystem , matrix (chemical analysis) , gas chromatography–mass spectrometry , adsorption , materials science , nanotechnology , organic chemistry , composite material
A capillary force filling microsystem consisting of a chip‐integrated solid‐phase microextraction (SMEC) array and a microdispenser for sample purification and trace enrichment of peptides is described. The microextraction array was loaded with solid‐phase media (50 μm Poros R2 beads) for purification and enrichment of proteomic samples. Samples bound to the SMEC were eluted in a volume of 200 nL. A piezo‐electric microdispenser was docked to the array and the samples bound to the SMEC were eluted in a volume of 200 nL using capillary forces. The purified and enriched samples were dispensed onto the matrix‐assisted laser desorption/ionization (MALDI) target, providing quality data from samples in the picomolar range. The nanoproteomic platform was compared to corresponding commercial preparation protocols, showing higher mass spectrometry (MS) signal intensities for peptides generated from an α‐casein digest. The platform was also elvaluated with regards to two‐dimensional (2‐D) gel‐derived protein digests from both fibroblast and epithelial target cells.