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Electrophoresis microchip fabricated by a direct‐printing process with end‐channel amperometric detection
Author(s) -
Tomazelli Coltro Wendell K.,
Fracassi da Silva José A.,
Torres da Silva Heron D.,
Richter Eduardo M.,
Furlan Rogério,
Angnes Lúcio,
do Lago Claudimir L.,
Mazo Luiz H.,
Carrilho Emanuel
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406091
Subject(s) - amperometry , polyester , lamination , capillary electrophoresis , materials science , microelectrode , electrophoresis , iodide , layer (electronics) , screen printing , chromatography , analytical chemistry (journal) , electrode , chemistry , nanotechnology , composite material , electrochemistry , organic chemistry
We describe the development of an electrophoresis microchip fabricated by a direct‐printing process, based on lamination of printed polyester films with end‐channel amperometric detection. The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester‐toner devices presented an electroosmotic flow (EOF) magnitude of ∼10 ‐5 cm 2 V ‐1 s ‐1 , which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser‐printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol·L ‐1 (135 amol) and 1.8 μmol·L ‐1 (486 amol) for iodide and ascorbate, respectively.