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New azo dyes as colored isoelectric point markers for isoelectric focusing in acidic pH region
Author(s) -
Štastná Miroslava,
Trávníček Martin,
Šlais Karel
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406088
Subject(s) - isoelectric point , isoelectric focusing , chemistry , colored , chromatography , electrophoresis , spectrophotometry , analytical chemistry (journal) , materials science , biochemistry , composite material , enzyme
Newly prepared azo compounds and several commercially available indicators were investigated for their applicability as colored isoelectric point (p I ) markers for isoelectric focusing (IEF) in the acidic range below pH 5. The majority of compounds described here can serve as primary standards since their p I values were determined by UV–VIS spectrophotometry independently IEF and direct measurement with a pH electrode. Subjected to gel IEF they show narrow and well‐observable zones of different colors. Finally, our work resulted in suggestion of a color ladder composed of p I markers covering the pH range from 1.5 to 4.7.