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Direct electrochemical detection of glucose in human plasma on capillary electrophoresis microchips
Author(s) -
Du Yan,
Yan Jilin,
Zhou Weihong,
Yang Xiuyong,
Wang Erkang
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406072
Subject(s) - capillary electrophoresis , chromatography , capillary action , electrophoresis , human plasma , chemistry , electrochemistry , plasma , analytical chemistry (journal) , materials science , electrode , physics , quantum mechanics , composite material
Abstract We developed an electrochemical detector on a hybrid chip for the determination of glucose in human plasma. The microchip system described in this paper consists of a poly(dimethylsiloxane) (PDMS) layer containing separation and injection channels and an electrode plate. The copper microelectrode is fabricated by selective electroless deposition. The fabrication of the decoupler is performed by platinum electrochemical deposition on the metal film formed by electroless deposition. Factors influencing the performance, including detection potential, separation field strength, and buffer concentration, were studied. The electrodes exhibited good stability and durability in the analytical procedures. Under optimized detection conditions, glucose responded linearly from 10 μ M to 1 m M . Finally, glucose in human plasma from three healthy individuals and two diabetics was successfully determined, giving a good prospect for a new clinical diagnostic instrument.