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Pyrimidine 5'‐nucleotidase activities detected in real samples by means of micellar electrokinetic chromatography
Author(s) -
Za Chiara,
Chiarelli Laurent R.,
Valentini Giovanna,
Perani Eleonora,
Annovazzi Laura,
Viglio Simona,
Iadarola Paolo
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406057
Subject(s) - micellar electrokinetic chromatography , electrokinetic phenomena , chromatography , 5' nucleotidase , chemistry , nucleotidase , electrophoresis , nucleoside , pyrimidine , substrate (aquarium) , enzyme , biochemistry , biology , ecology
A micellar electrokinetic chromatographic method that allows simultaneous determination of both nucleotidase and transferase activities of cytosolic 5'‐nucleotidase III is presented. This electrophoretic approach was successfully applied to human erythrocyte lysates to monitor the enzyme activities indicated above, using either physiological substrate or the nucleoside analogue 3'‐azido‐3'‐deoxythymidine as the phosphate acceptor.