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Microemulsion electrokinetic chromatography for the analysis of acrylamide in food
Author(s) -
Bermudo Elisabet,
RuizCalero Victoria,
Puignou Lluis,
Galceran Maria T.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406044
Subject(s) - microemulsion , acrylamide , chromatography , chemistry , electrokinetic phenomena , micellar electrokinetic chromatography , sodium dodecyl sulfate , aqueous solution , detection limit , sodium , propanol , alcohol , methanol , calibration curve , butanol , polyacrylamide , analytical chemistry (journal) , ethanol , pulmonary surfactant , organic chemistry , polymer , polymer chemistry , copolymer , biochemistry
The influence of microemulsion electrokinetic chromatography (MEEKC) operating conditions, such as the type of water‐immiscible alcohol, aqueous phosphate buffer concentration, pH, as well as the addition of methanol and 2‐propanol, on acrylamide migration has been studied. These parameters have been optimized taking into account the presence of matrix signals, in order to avoid the interference of these peaks in acrylamide determination. The best separations were achieved using a microemulsion consisting of 0.8% m/v n ‐amyl alcohol, 3.3% m/v sodium dodecyl sulfate (SDS), 6.6% m/v 1‐butanol, and 89.3% m/v 40 m M phosphate buffer at pH 6.5 working at 15 kV in uncoated silica capillaries. Linear calibration curves over the range studied (1.25–125 μg·mL ‐1 ), the detection limit (0.70 μg·mL ‐1 ), and both run‐to‐run (up to 3.4% for concentration and 1.6% for time values) and day‐to‐day precision (lower than 11.6% for concentration) have been established. Finally, the applicability of the MEEKC method developed has been demonstrated by analyzing levels of acrylamide present in samples of home‐made French fries.