Premium
Whole‐column fluorescence‐imaged capillary electrophoresis
Author(s) -
Wu XingZheng,
Pawliszyn Janusz
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406043
Subject(s) - fluorescence , laser induced fluorescence , chromatography , capillary action , chemistry , fluorescein , fluorescein isothiocyanate , analytical chemistry (journal) , capillary electrophoresis , electrophoresis , laser , materials science , optics , physics , composite material
An axially illuminating whole‐column fluorescence imaging capillary electrophoresis (CE) experimental setup was developed. A 6 cm long Teflon tube with an inside diameter (ID) of 42 μm was used as separation column. Excitation light of 488 nm from Ar + laser was introduced to one end of the separation column by an optical fiber. The excitation light propagated inside the separation column by total internal reflection, since the refractive index of the buffer solution was larger than that of the Teflon tube. The fluorescence from the whole separation column was imaged with a charge‐coupled device (CCD) camera. Fluorescent compounds such as fluorescein isothiocyanate (FITC), 5‐carboxyfluorescein, and FITC‐labeled protein were used to test the basic performance of the experimental setup. Experimental results illustrate that the whole‐column‐fluorescence imaging CE is a fast and sensitive separation method for fluorescent compounds and fluorescent‐labeled proteins. Furthermore, it could be used for simple, fast, and easy comparisons of the resistance to photodegradation for various fluorescent compounds.