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Improved resolution power of electrophoretic fractionation of DNA using a voltage gradient “up and down” application
Author(s) -
Bellavia Daniele,
Sanfilippo Rosalia,
Sisino Giorgia,
Palmeri Laura,
Barbieri Rainer
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406032
Subject(s) - fractionation , electrophoresis , resolution (logic) , chromatography , voltage , chemistry , analytical chemistry (journal) , computer science , electrical engineering , engineering , artificial intelligence
The improved resolution power of electrophoretic fractionation of DNA in a wide range of molecular masses is demonstrated using an “up and down” application of voltage gradient gel electrophoresis (VGGE). This application also allows separation of different DNA fragments which are poorly fractionated in conventional electrophoresis.