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Optimization and validation of an enantioselective method for a chiral drug with eight stereo‐isomers in capillary electrophoresis
Author(s) -
Jimidar M. Ilias,
Vennekens Tom,
Van Ael Willy,
Redlich Dirk,
De Smet Maurits
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406031
Subject(s) - capillary electrophoresis , chromatography , enantioselective synthesis , robustness (evolution) , capillary action , chemistry , response surface methodology , biological system , materials science , organic chemistry , biochemistry , composite material , gene , catalysis , biology
Highly selective capillary electrophoresis (CE) screening methods were applied to find a satisfactory separation of a chiral drug with eight stereoisomeric compounds. The initial separation conditions were further optimized using response surface modelling by applying a Box‐Behnken experimental design. This approach resulted in a rapid and efficient optimization of the buffer concentration, the concentration of two cyclodextrins, and the run voltage, in order to obtain final separation conditions of the method. Further optimization and validation of the system in terms of sensitivity and robustness resulted in a method that is suitable for quality control release purposes.