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Subsecond chiral separations on a microchip
Author(s) -
Piehl Natalia,
Ludwig Martin,
Belder Detlev
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200406028
Subject(s) - chemistry , amino acid , phenylalanine , enantiomer , norleucine , methionine , chromatography , tryptophan , stereochemistry , biochemistry
Fast chiral separation of DNS‐amino acids could be realized using microchip electrophoresis with fluorescence detection. For this purpose, highly sulfated cyclodextrins (HS‐γ‐CD) were used as chiral selectors enabling high selectivity. Even subsecond separation of DNS‐tryptophan, DNS‐norleucine, DNS‐phenylalanine, DNS‐methionine, and DNS‐aspartic acid could be achieved. Baseline separation could be accomplished within 720 ms, which is the fastest separation of enantiomers reported to date. A more complex mixture consisting of three chiral DNS‐amino acids could be separated within 3.3 s utilizing a separation length of only 7 mm and an electrical field strength of 2012 V/cm.