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Comparative study on the chiral separation of phenyl alcohols by capillary electrophoresis and liquid chromatography
Author(s) -
Zhang YuPing,
Lee KwangPill,
Kim SangHo,
Choi SeongHo,
Gopalan Anantha I.,
Yuan ZhuoBin
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200405969
Subject(s) - capillary electrophoresis , chromatography , chemistry , electrophoresis , resolution (logic) , high performance liquid chromatography , analyte , capillary action , selectivity , capillary electrochromatography , analytical chemistry (journal) , materials science , organic chemistry , artificial intelligence , composite material , catalysis , computer science
Abstract Structurally related phenyl alcohols were separated by capillary electrophoresis and liquid chromatography. A statistical experimental design was used in order to optimize the main electrophoretic parameters such as pH, concentration of selector and separation voltage in capillary electrophoresis (CE). Response surfaces were derived using the mathematical model and used for a selection of the optimal experimental conditions. Concentration of the chiral selector, the distance between the aromatic group and asymmetric center of the analytes, were identified as the factors influencing the complexation, selectivity and resolution. Experiments were also performed by high‐performance liquid chromatography (HPLC) and the results of CE and HPLC were compared.