Premium
Simultaneous determination of polyamines and catecholamines in PC‐12 tumor cell extracts by capillary electrophoresis with laser‐induced fluorescence detection
Author(s) -
Du Ming,
Flanigan Virgil,
Ma Yinfa
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200405896
Subject(s) - capillary electrophoresis , spermidine , chemistry , chromatography , detection limit , laser induced fluorescence , derivatization , fluorescein isothiocyanate , fluorescence , putrescine , spermine , electrophoresis , mass spectrometry , biochemistry , enzyme , physics , quantum mechanics
A method to detect polyamines and catecholamines in PC‐12 tumor cell extracts by capillary electrophoresis with laser‐induced fluorescence detection (CE‐LIF) is described for the first time. Both derivatization conditions and buffer concentrations and pH were optimized. Under optimized conditions the polyamines (putresine, spermine, spermidine) and catecholamines (dopamine, norepinephrine, epinephrine, serotonin) were derivatized with fluorescein isothiocyanate and separated at 25 kV in a fused‐silica capillary (50 μm ID×40 cm) with 0.1 M borate, pH 9.0, in less than 18 min. The influence of running buffer conditions, such as buffer pH and concentrations, were also investigated. Linearity of the analytes ranged from 0.05 to 1.0 μmol/L, and the detection limit (S/N = 3 ) ranged from 0.03 to 2.50 nmol/L. The concentrations of polyamines and catecholamines in PC‐12 tumor cell extracts were determined with this method.