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Selectivity of quadruplex DNA stationary phases toward amino acids in homodipeptides and alanyl dipeptides
Author(s) -
Vo Trang U.,
McGown Linda B.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200405865
Subject(s) - oligonucleotide , aptamer , chemistry , capillary electrophoresis , thymine , guanine , dna , thrombin , covalent bond , g quadruplex , stereochemistry , biophysics , crystallography , chromatography , biochemistry , nucleotide , microbiology and biotechnology , organic chemistry , platelet , biology , gene , immunology
Series of dipeptides, including homodipeptides and alanyl dipeptides, were separated using quadruplex (G‐quartet) DNA stationary phases in open‐tubular capillary electrochromatography (OTCEC). The stationary phases were constructed by covalently attaching the DNA oligonucleotides to the inner capillary surface. Three different G‐quartet forming oligonucleotides were investigated: the two‐plane G‐quartet forming thrombin‐binding aptamer, the four‐plane analogue of the thrombin‐binding aptamer, and a two‐plane oligonucleotide identical to the thrombin‐binding aptamer except for the replacement of the guanine by thymine in the central bridging loop of the G‐quartet structure. Results were compared with results obtained using capillary electrophoresis on a bare capillary and OTCEC using an oligonucleotide with the same base composition as the thrombin‐binding aptamer but in a different sequence that does not allow G‐quartet formation as the stationary phase.