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Micellar electrokinetic chromatography of enkephalin‐related peptides with laser‐induced fluorescence detection
Author(s) -
Huang Ying,
Duan Jianping,
Chen Qiaoren,
Chen Guonan
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200405810
Subject(s) - derivatization , chromatography , chemistry , micellar electrokinetic chromatography , detection limit , sodium dodecyl sulfate , capillary electrophoresis , laser induced fluorescence , fluorescence , sodium , analyte , fluorescein isothiocyanate , bodipy , high performance liquid chromatography , organic chemistry , physics , quantum mechanics
Eight enkephalin‐related peptides were derivatized using fluorescein isothiocyanate (FITC), and the derivatization products were separated and detected by capillary electrophoresis with laser‐induced fluorescence detection. The optimum molar ratio of FITC and peptide for derivatization was found to be 40:1, and 5 mmol/L sodium borate buffer (pH 9.2) was selected as derivatization media in order to get the high efficiency. Enkephalin‐related peptides were completely separated in the pH range of 10.51–10.60 in a running buffer consisting of 100 mmol/L sodium borate and 60 mmol/L sodium dodecyl sulfate (SDS). The detection limit for these eight enkephalins ranged from 0.18 to 2.25 nmol/L, and the linear response range was 1.0×10 −6 to 1.0×10 −9 mol/L with correlation coefficients between 0.9947–0.9988. A separation efficiency as high as 380 000 theoretical plates could be obtained for these analytes.