Premium
Study on pharmacokinetics and tissue distribution of norvancomycin in rats by CE with electrochemical detection
Author(s) -
Zhou Tianshu,
Wu Fang,
Shi Guoyue,
Wang Aifang,
Hu Qin,
Wang Xiaochun,
Fang Yuzhi
Publication year - 2006
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200400774
Subject(s) - pharmacokinetics , tissue distribution , detection limit , chromatography , chemistry , distribution (mathematics) , kidney , spleen , pharmacology , medicine , mathematical analysis , mathematics
In this paper, we developed a sensitive and simple method to study the pharmacokinetics and tissue distribution of norvancomycin (NVCM) in experimental animals by using CE with electrochemical detection. Pharmacokinetics investigation was performed by the collection of blood samples at timed intervals following administration of NVCM. Pharmacokinetic parameters were calculated by the 3P87 pharmacokinetic program. The elimination half‐life of NVCM was 42.4742 min with a clearance rate of 0.0233 mL·kg −1 ·min −1 . Additionally, drug distribution was studied by measuring the NVCM levels in kidney, lung, stomach, intestine, spleen, heart, liver, and cerebrum. Electrophoresis conditions such as buffer solution, working potential, separation voltage, and sampling time were also discussed. The linear range was from 0.8 to 540 μg/mL with a correlation coefficient of 0.9991. The detection limit was 0.3 μg/mL. This method was for the first time applied to study the pharmacokinetics and tissue distributions of NVCM in experimental animals.