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Simultaneous determination of anthraquinones in Rhubarb by pressurized liquid extraction and capillary zone electrophoresis
Author(s) -
Gong Yuan X.,
Li Shao P.,
Wang Yi T.,
Li Peng,
Yang Feng Q.
Publication year - 2005
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200400001
Subject(s) - anthraquinones , capillary electrophoresis , chromatography , extraction (chemistry) , chemistry , capillary action , electrophoresis , materials science , biology , botany , composite material
Rhubarb, a well‐known Chinese herbal medicine, is also used in Europe and other places of the world. Anthraquinones derivatives are thought to be the major active components. A pressurized liquid extraction (PLE) and capillary zone electrophoresis (CZE) separation were developed for simultaneous determination of five anthraquinones including aloe‐emodin, emodin, chrysophanol, physcion, and rhein in Rhubarb. The effects of the experimental variables on PLE and CZE have been optimized. The optimum conditions of PLE were: solvent, methanol; temperature, 140°C; particle size, 0.13–0.2 mm; static extraction time, 5 min; pressure, 1500 psi; and one extraction. The best separation of the five anthraquinones could be obtained using 50 m M borate buffer (pH 8.2) containing 25% isopropyl alcohol and 25% acetontrile as modifier, while the separation voltage was 25 kV and the temperature was at 20°C. The method developed is accurate, simple, and reproducible, and could be used for quality control of Rhubarb and its medical preparations.