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Identification of a specifically expressed modified form of novel PSP‐94 protein in the secretion of benign prostatic hyperplasia
Author(s) -
Xu Kexin,
Wang Xianghong,
Ling Mingtat,
Lee Davy T. W.,
Fan Tianyong,
Chan Franky L.,
Xuan James J. W.,
Tsao SaiWah,
Wong YongChuan
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390167
Subject(s) - hyperplasia , gene isoform , secretion , secretory protein , prostate , alternative splicing , gene , rna splicing , biology , homology (biology) , microbiology and biotechnology , endocrinology , chemistry , medicine , biochemistry , cancer , rna
Using two‐dimensional gel electrophoresis, we investigated the profile of prostatic secretory proteins in human expressed prostatic secretion (EPS) from benign prostate hyperplasia (BPH) patients and compared the patterns with normal controls. We identified three specifically expressed proteins, including prostate secretory protein 61 (PSP61), in EPS from benign BPH patients but absent in normal controls. In addition, we found that PSP61 was a modified isoform of the well‐documented PSP‐94, which had a perfect matching (100% homology) to the first 61 amino acids of the PSP‐94 protein but with a deleted C ‐terminus. This shortened PSP61 was not due to alternative splicing of the PSP‐94 gene at transcription level. Our results provide first evidence on the possibility of using PSP61 as a specific biological marker for diagnosis of BPH.