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Quantitative analysis of aglycone quercetin in mulberry leaves ( Morus alba L.) by capillary zone electrophoresis
Author(s) -
Suntornsuk Leena,
Kasemsook Saowapak,
Wongyai Surapote
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390159
Subject(s) - aglycone , rutin , chromatography , chemistry , quercetin , detection limit , capillary electrophoresis , quercitrin , kaempferol , gallic acid , glycoside , stereochemistry , biochemistry , antioxidant
Abstract A capillary zone electrophoresis method was established for analysis of aglycone quercetin in mulberry leaves (Morus alba L.). The influence of, e.g. , background electrolyte concentrations and pH, surfactant concentrations, organic solvents, temperature, and voltage on the separation of aglycone quercetin, rutin, quercitrin, kaempferol, catechin, and gallic acid was systematically investigated. The optimum condition providing baseline separation of all compounds within 16.5 min was obtained in 150 m M boric acid (pH 10.0) using a fused‐silica capillary with an effective length of 42.5 cm (50 μm inner diameter), temperature of 32°C, and voltage of 15 kV. Method assessment was performed by standard addition method using rutin as an internal standard. Linearity of the method was excellent ( r 2 > 0.999) over the concentration tested (40–160 μg/mL). The relative standard deviations (%RSDs) from injection, intraday, and interday precision were less than 2.5%. Recoveries were good (≈ 100.0%,%RSD = 0.8%) with a limit of detection (LOD) and limit of quantitation (LOQ) of 0.86 and 3.16 μg/mL (%RSD = 1.8%), respectively. The aglycone quercetin found in the mulberry leaves was 0.452 g/100 g (%RSD = 0.6%) on dry weight.