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Simultaneous detection of S ‐adenosylmethionine and S ‐adenosylhomocysteine in mouse and rat tissues by capillary electrophoresis
Author(s) -
Uthus Eric O.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390157
Subject(s) - capillary electrophoresis , chromatography , detection limit , perchloric acid , chemistry , resolution (logic) , organic chemistry , artificial intelligence , computer science
A capillary electrophoresis method for the determination of S ‐adenosylmethionine (SAM) and S ‐adenosylhomocysteine (SAH) in rat liver and kidney and mouse liver is described. The method can also be used to determine SAM in whole blood. The method provides rapid (approximately 16 min sample to sample) resolution of both compounds in perchloric extracts of tissues. Separation was performed by using an uncoated 50 μm ID capillary with 60 cm total length (50 cm to the detector window). Samples were separated at 22.5 kV and the separation running buffer was 200 m M glycine pH 1.8 (with HCl). The method compares favorably to HPLC methods ( r 2 = 0.994 for SAM, r 2 = 0.998 for SAH) and has a mass detection limit of about 10 fmol for both SAM and SAH at a signal‐to‐noise ratio of 3. The method is linear over ranges of 1–100 μ M SAM and 1–250 μ M SAH. This method can be used to determine tissue concentrations of SAM and SAH, two metabolites that can provide insight into many biological processes.