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Determination of lysergic acid diethylamide (LSD) in mouse blood by capillary electrophoresis/ fluorescence spectroscopy with sweeping techniques in micellar electrokinetic chromatography
Author(s) -
Fang Ching,
Liu JuTsung,
Chou ShiuHuey,
Lin ChengHuang
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390119
Subject(s) - micellar electrokinetic chromatography , chromatography , chemistry , capillary electrophoresis , sodium dodecyl sulfate , electrokinetic phenomena , fluorescence spectroscopy , pulmonary surfactant , lysergic acid diethylamide , electrophoresis , micelle , fluorescence , biochemistry , organic chemistry , physics , receptor , quantum mechanics , serotonin , aqueous solution
The separation and on‐line concentration of lysergic acid diethylamide (LSD) in mouse blood was achieved by means of capillary electrophoresis/fluorescence spectroscopy using sodium dodecyl sulfate (SDS) as the surfactant. Techniques involving on‐line sample concentration, including sweeping micellar electrokinetic chromatography (sweeping‐MEKC) and cation‐selective exhaustive injection‐sweep‐micellar electrokinetic chromatography (CSEI‐sweep‐MEKC) were applied; the optimum on‐line concentration and separation conditions were determined. In the analysis of an actual sample, LSD was found in a blood sample from a test mouse (0.1 mg LSD fed to a 20 g mouse; ∼1/10 to the value of LD 50 ). As a result, 120 and 30 ng/mL of LSD was detected at 20 and 60 min, respectively, after ingestion of the doses.