Premium
On‐line identification of lysergic acid diethylamide (LSD) in tablets using a combination of a sweeping technique and micellar electrokinetic chromatography/77 K fluorescence spectroscopy
Author(s) -
Fang Ching,
Liu JuTsung,
Lin ChengHuang
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390118
Subject(s) - micellar electrokinetic chromatography , chromatography , lysergic acid diethylamide , chemistry , electrokinetic phenomena , mass spectrometry , fluorescence spectroscopy , gas chromatography , lysergic acid , analytical chemistry (journal) , fluorescence , electrophoresis , stereochemistry , biochemistry , physics , receptor , quantum mechanics , serotonin
This work describes a novel method for the accurate determination of lysergic acid diethylamide (LSD) in tablets. A technique involving sweeping‐micellar electrokinetic chromatography (MEKC) was used for the initial on‐line concentration and separation, after which a cryogenic molecular fluorescence experiment was performed at 77 K. Using this approach, not only the separation of LSD from the tablet extract was achieved, but on‐line spectra were readily distinguishable and could be unambiguously assigned. The results are in agreement with analyses by gas chromatography‐mass spectrometry (GC‐MS). Thus, this method, which was found to be accurate, sensitive and rapid, has the potential for use as a reliable complementary method to GC‐MS in such analyses.