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Capillary electrophoresis coupled to time of flight‐mass spectrometry of therapeutic peptide hormones
Author(s) -
SanzNebot Victoria,
Benavente Fernando,
Balaguer Elvira,
Barbosa José
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390111
Subject(s) - capillary electrophoresis , chromatography , repeatability , capillary action , mass spectrometry , chemistry , capillary electrophoresis–mass spectrometry , electrospray , reproducibility , electrolyte , analytical chemistry (journal) , electrospray ionization , materials science , electrode , composite material
We have established a method for separation and characterization of a series of peptide hormones of pharmaceutical interest and wide therapeutical use by capillary electrophoresis‐electrospray‐mass spectrometry (CE‐ES‐MS) using a sheath flow interface. Several parameters were systematically investigated, such as concentration of the electrolyte, organic solvent and sheath liquid composition, gas flow rates and capillary position. Moreover, limits of detection, linearity, repeatability and day‐to‐day reproducibility of the proposed method were studied in order to obtain the main quality parameters.