Separation of homologues and isomers of linear alkylbenzenesulfonates by capillary electrophoresis with sodium dodecyl sulfate, carboxylic acids and bile salts

The ability of several anionic compounds, including carboxylic and dicarboxylic acids, sodium dodecyl sulfate (SDS), and sodium deoxycholate (SDC) and other bile salts, to separate the C 10 –C 13 homologues and the corresponding 20 positional isomers of linear alkylbenzenesulfonates (LAS) by capillary electrophoresis was studied. Up to 19 peaks and a shoulder were observed with a background electrolyte (BGE) containing 10 m M phosphate (pH 6.8), 30% acetonitrile and 40 m M SDS, and 18 peaks were obtained with a BGE containing 10 m M borate (pH 9), 40% ethanol and 40 m M palmitic acid (PA). Resolution increased with the alkyl chain length of the carboxylic acid. Dicarboxylic acids with a short alkyl chain, as azelaic acid, were useful to separate the homologues without distinguishing between the isomers. Up to 16 peaks and a shoulder were distinguished with SDC. Resolution decreased with the other bile salts. The 6‐C 11 /5‐C 11 isomer pair was better resolved with SDC than with SDS, and the 2‐C 12 isomer was isolated using both PA and SDC, but not with SDS. Only the 7‐C 13 /6‐C 13 pair could not be resolved with any of the discriminating agents used.