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Separation of homologues and isomers of linear alkylbenzenesulfonates by capillary electrophoresis with sodium dodecyl sulfate, carboxylic acids and bile salts
Author(s) -
HerreroMartínez José Manuel,
SimóAlfonso Ernesto F.,
RamisRamos Guillermo
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390081
Subject(s) - chemistry , sodium dodecyl sulfate , carboxylic acid , capillary electrophoresis , alkyl , structural isomer , chromatography , sodium , acetonitrile , electrolyte , electrophoresis , stereochemistry , organic chemistry , electrode
The ability of several anionic compounds, including carboxylic and dicarboxylic acids, sodium dodecyl sulfate (SDS), and sodium deoxycholate (SDC) and other bile salts, to separate the C 10 –C 13 homologues and the corresponding 20 positional isomers of linear alkylbenzenesulfonates (LAS) by capillary electrophoresis was studied. Up to 19 peaks and a shoulder were observed with a background electrolyte (BGE) containing 10 m M phosphate (pH 6.8), 30% acetonitrile and 40 m M SDS, and 18 peaks were obtained with a BGE containing 10 m M borate (pH 9), 40% ethanol and 40 m M palmitic acid (PA). Resolution increased with the alkyl chain length of the carboxylic acid. Dicarboxylic acids with a short alkyl chain, as azelaic acid, were useful to separate the homologues without distinguishing between the isomers. Up to 16 peaks and a shoulder were distinguished with SDC. Resolution decreased with the other bile salts. The 6‐C 11 /5‐C 11 isomer pair was better resolved with SDC than with SDS, and the 2‐C 12 isomer was isolated using both PA and SDC, but not with SDS. Only the 7‐C 13 /6‐C 13 pair could not be resolved with any of the discriminating agents used.