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Differences in capillary electrophoresis profiles of urinary and recombinant erythropoietin
Author(s) -
de Frutos Mercedes,
Cifuentes Alejandro,
DiezMasa Jose C.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390080
Subject(s) - capillary electrophoresis , recombinant dna , erythropoietin , chromatography , urinary system , chemistry , capillary action , electrophoresis , biology , biochemistry , materials science , endocrinology , gene , composite material
Different profiles were obtained by capillary zone electrophoresis (CZE) of human erythropoietin (EPO) of recombinant and urinary origin. To unambiguously detect doping by EPO, direct methods able to determine the presence of the drug itself in a physiological fluid are required. Since the host cell line used for EPO production influences its glycosylation, the carbohydrate distribution of natural human EPO may be different from that of recombinant EPO. The different content in sialic acid groups between recombinant and endogenous EPO provide a basis for their distinction by CZE.