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Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detection
Author(s) -
Coufal Pavel,
Zuska Jiří,
van de Goor Tom,
Smith Vonda,
Gaš Bohuslav
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390079
Subject(s) - capillary electrophoresis , chromatography , analyte , chemistry , electrophoresis , electrokinetic phenomena , electrolyte , isotachophoresis , amino acid , capillary action , detection limit , reproducibility , materials science , biochemistry , electrode , composite material
Twenty underivatized essential amino acids were separated using capillary zone electrophoresis and consequently detected with contactless conductivity detection (CCD). A simple acidic background electrolyte (BGE) containing 2.3 M acetic acid and 0.1% w/w hydroxyethylcellulose (HEC) allowed the electrophoretic separation and sensitive detection of all 20 essential amino acids in their underivatized cationic form. The addition of HEC to the BGE suppressed both, electroosmotic flow and analyte adsorption on the capillary surface resulting in an excellent migration time reproducibility and a very good analyte peak symmetry. Additionally, the HEC addition significantly reduced the noise and long‐term fluctuations of the CCD baseline. The optimized electrophoretic separation method together with the CCD was proved to be a powerful technique for determination of amino acid profiles in various natural samples, like beer, yeast, urine, saliva, and herb extracts.