Premium
Indirect laser‐induced fluorescence detection for capillary electrophoresis using a frequency‐doubled diode laser
Author(s) -
Ragozitalia,
Pütz Michael,
Faubel Werner,
Pyell Ute
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390064
Subject(s) - chemistry , capillary electrophoresis , detection limit , analytical chemistry (journal) , chromatography , laser induced fluorescence , fluorescence , nitrite , ion chromatography , chlorate , thiocyanate , hydroxide , chloride , nitrate , inorganic chemistry , physics , organic chemistry , quantum mechanics
A blue (452 nm) frequency‐doubled diode laser with a quasi‐cw optical output power of 10 μW is used for indirect laser‐induced fluorescence detection in combination with the capillary electrophoretic separation of inorganic anions. As fluorescing probe ion the anion of 8‐hydroxypyrene‐1,3,6‐trisulfonic acid (HPTS) was selected having an absorption maximum of 454 nm in alkaline medium. Employing a capillary coated with linear acrylamide, baseline separation of eight inorganic anions was possible within 5 min. With a separation buffer containing 50 μmol·L ‐1 HPTS and 10 mmol·L ‐1 lysine the limits of detection for sulfate, nitrite, nitrate, azide, thiocyanate, and chlorate were between 0.9 and 4.7 μmol·L ‐1 . Separation of chloride and sulfate was achieved by adding 0.25 mmol·L ‐1 calcium hydroxide to the separation buffer. Inorganic anions in several mineral and tap water samples have been determined with the technique developed and results are compared to data obtained by ion chromatography in combination with conductivity detection after conductivity suppression.