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Stacking phenomena in electromigration: From basic principles to practical procedures
Author(s) -
Urbánek Marek,
Křivánková Ludmila,
Boček Petr
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390056
Subject(s) - stacking , electromigration , capillary electrophoresis , analyte , isotachophoresis , electrokinetic phenomena , conductivity , micellar electrokinetic chromatography , materials science , analytical chemistry (journal) , chemistry , chromatography , nanotechnology , electrolyte , electrode , organic chemistry , composite material
The review describes principles of procedures and techniques used in capillary zone electrophoresis for enhancement of sensitivity that are based on increasing analyte mass in its zone during the electromigration process, for which the term stacking is generally used. Attention is paid to intrinsic stacking in samples with low conductivity, transient isotachophoretic stacking applied in samples with high conductivity, and sweeping in micellar electrokinetic chromatography. Principles of these stacking schemes are explained, new procedures and instrumental arrangements are discussed, and all contributions involving stacking principles that have been published since the year 2000 are surveyed.