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An exactly solvable Ogston model of gel electrophoresis: X. Application to high‐field separation techniques
Author(s) -
Gauthier Michel G.,
Slater Gary W.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390053
Subject(s) - electric field , electrophoresis , nonlinear system , microfluidics , field (mathematics) , trapping , separation (statistics) , physics , chemistry , materials science , nanotechnology , chromatography , computer science , mathematics , quantum mechanics , ecology , machine learning , pure mathematics , biology
Abstract Recently, we generalized our lattice model of gel electrophoresis to study the net velocity of particles being pulled by a high‐intensity electric field through an arbitrary distribution of immobile obstacles (Gauthier, M. G., Slater, G. W., J. Chem. Phys. 2002, 117 , 6745–6756). In this article, we show how the high‐field version of our model can be used to compare the velocity of particles with different electric charges and/or physical sizes. We then investigate specific two‐dimensional distributions of obstacles that can be used to separate particles, e.g. , in a microfluidic device. More precisely, we compare the velocity of differently charged or sized analytes in sieving, trapping and deflecting systems to model various electrophoretic separation techniques. In particular, we study the nonlinear effects present in ratchet systems and how they can be combined with time‐asymmetric pulsed fields to provide new modes of separation.