An interface chip connection between capillary electrophoresis and thermal lens microscope | Zendy

Uchiyama Kenji | Zendy; Hibara Akihide | Zendy; Sato Kiichi | Zendy; Hisamoto Hideaki | Zendy; Tokeshi Manabu | Zendy; Kitamori Takehiko | Zendy

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An interface chip connection between capillary electrophoresis and thermal lens microscope

Author(s) -

Uchiyama Kenji,

Hibara Akihide,

Sato Kiichi,

Hisamoto Hideaki,

Tokeshi Manabu,

Kitamori Takehiko

Publication year - 2003

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.200390011

Subject(s) - capillary electrophoresis , absorbance , detection limit , microchannel , microscope , lens (geology) , capillary action , materials science , analytical chemistry (journal) , chromatography , electrophoresis , chemistry , optics , nanotechnology , composite material , physics

A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused‐silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8×10 ‐7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE‐TLM was estimated as 2.4×10 ‐8 M , which was 100 times lower than that of conventional absorbance detection.

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