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An interface chip connection between capillary electrophoresis and thermal lens microscope
Author(s) -
Uchiyama Kenji,
Hibara Akihide,
Sato Kiichi,
Hisamoto Hideaki,
Tokeshi Manabu,
Kitamori Takehiko
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390011
Subject(s) - capillary electrophoresis , absorbance , detection limit , microchannel , microscope , lens (geology) , capillary action , materials science , analytical chemistry (journal) , chromatography , electrophoresis , chemistry , optics , nanotechnology , composite material , physics
A thermal lens microscope (TLM) detection of capillary electrophoresis (CE) utilizing microchip technology was developed. Fused‐silica capillaries with an inner diameter of 50 μm were directly connected to a microchannel in a microchip. The detection limit by TLM was estimated as 2.8×10 ‐7 absorbance by measuring pure water. The detection limit of derivatized amino acids determined by CE‐TLM was estimated as 2.4×10 ‐8 M , which was 100 times lower than that of conventional absorbance detection.