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Characterization of pharmaceutical drugs by a modified nonaqueous capillary electrophoresis – mass spectrometry method
Author(s) -
PeriOkonny Unita L.,
Kenndler Ernst,
Stubbs R. John,
Guzman Norberto A.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200390006
Subject(s) - chromatography , chemistry , capillary electrophoresis , analyte , ammonium formate , mass spectrometry , resolution (logic) , capillary electrophoresis–mass spectrometry , electrolyte , high performance liquid chromatography , electrospray ionization , electrode , artificial intelligence , computer science
A simple method for the separation and characterization of a group of nine basic compounds, comprising seven tricyclic antidepressant and two bronchodilator drugs, by nonaqueous capillary electrophoresis (NACE) employing ultraviolet and mass spectrometry detection is described. After optimization of the electrophoresis separation conditions, including the compositions of the electrolyte and the organic solvent, a reliable separation of all nine basic analytes was achieved in 80 m M ammonium formate dissolved in a methanol‐acetonitrite (80:20 v/v) mixture, having an apparent pH of 8.7. The volatile nonaqueous electrolyte system used with a normal electroosmotic flow polarity also provided an optimal separation condition for the characterization of the analytes by mass spectrometry. When results were compared with reversed‐phase gradient and isocratic high‐performance liquid chromatography (HPLC) methods, the NACE method provided greater efficiency, achieving baseline resolution for all nine basic compounds in less than 30 min. The NACE method is suitable for use as a routine procedure for the rapid separation and characterization of basic compounds and is a viable alternative to HPLC for the separation of a wide range of pharmaceutical drugs.