Premium
Evaluation and validation of the ABI 3700, ABI 3100, and the MegaBACE 1000 capillary array electrophoresis instruments for use with short tandem repeat microsatellite typing in a forensic environment
Author(s) -
Koumi Pieris,
Green Helen E.,
Hartley Susan,
Jordan Darren,
Lahec Sharon,
Livett Richard J.,
Tsang Kam W.,
Ward David M.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305976
Subject(s) - capillary electrophoresis , microsatellite , multiplex , str analysis , dna sequencer , chromatography , analytical chemistry (journal) , biology , dna , dna sequencing , chemistry , genetics , gene , allele
The demand for high‐throughput DNA profiling has increased with the introduction of national DNA databases and has led to the development of automated methods of short tandem repeat (STR) profile production; however, a potential bottleneck still exists at the gel electrophoresis stage. Capillary electrophoresis sequencers capable of processing 96 samples with considerably reduced manual intervention are now available. In this paper, we compare the ABI Prism 377 slab‐gel sequencer currently used by the Forensic Science Service with three leading capillary array electrophoresis instruments: the ABI Prism 3700, the Amersham MegaBACE 1000 and the 16‐capillary ABI Prism 3100. We describe the experiments used to evaluate and validate these platforms for forensic use with the STR multiplex Amp f /STR SGMplus [1, 2], along with comparative data from the ABI Prism 377 sequencer.