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Gene expression analysis of an integrin family of genes by systematic multiplex reverse transcription‐polymerase chain reaction
Author(s) -
Yamamoto Miyako,
Yamamoto Ami,
Leung Patricia C.,
Yamamoto Fumiichiro
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305952
Subject(s) - rna splicing , gene , biology , alternative splicing , reverse transcriptase , microbiology and biotechnology , reverse transcription polymerase chain reaction , gene expression , polymerase chain reaction , integrin , multiplex polymerase chain reaction , messenger rna , genetics , rna , cell
We have established the systematic multiplex reverse transcription‐polymerase chain reaction (SM RT‐PCR) system of 26 members of the integrin family of genes, and used this system to investigate their expression in 25 different kinds of human adult tissues. A hierarchical clustering analysis of the tissue expression data illustrated clustering of functionally related tissues. Although the correlation was weak, bone marrow, thymus, and spleen were clustered, potentially confirming the role of certain integrin molecules in the immune response. We also investigated changes in integrin gene expression in cancer. Several differences were observed between normal and tumor, as well as between normal and cancer cell lines. The SM RT‐PCR experiments for the study of alternative splicing showed that most of the integrin genes analyzed exhibited fixed ratios of differentially spliced transcripts probably due to a tissue‐independent mechanism of splicing predetermined by the nucleotide sequences around the splicing donor and acceptor sites.