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Drug‐human serum albumin binding studied by capillary electrophoresis with electrochemiluminescence detection
Author(s) -
Zhao Xiaocui,
You Tianyan,
Liu Jifeng,
Sun Xiuhua,
Yan Jilin,
Yang Xiurong,
Wang Erkang
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305930
Subject(s) - capillary electrophoresis , electrochemiluminescence , human serum albumin , chemistry , ruthenium , chromatography , drug , bovine serum albumin , electrophoresis , serum albumin , tris , plasma protein binding , detection limit , biochemistry , pharmacology , medicine , catalysis
A new technique for investigating drug‐protein binding was developed employing capillary electrophoresis (CE) coupled with tris(2,2'‐bipyridyl) ruthenium(II) [Ru(bpy) 3 2+ ] electrochemiluminescence (ECL) (CE‐ECL) detection after equilibrium dialysis. Three basic drugs, namely pridinol, procyclidine and its analogue trihexyphenidyl, were successfully separated by capillary zone electrophoresis with end‐column Ru(bpy) 3 2+ ECL detection. The relative drug binding to human serum albumin (HSA) for each single drug as well as for the three drugs binding simultaneously was calculated. It was found that the three antiparkinsonian drugs compete for the same binding site on HSA. This work demonstrated that Ru(bpy) 3 2+ CE‐ECL can be a suitable technique for studying drug‐protein binding.