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S ‐Adenosyl methionine/ S ‐adenosyl‐ L ‐homocysteine ratio determination by capillary electrophoresis employed as a monitoring tool for the antiviral effectiveness of adenosine analogs
Author(s) -
Sbrana Elena,
Bramanti Emilia,
Spinetti Maria C.,
Raspi Giorgio
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305851
Subject(s) - capillary electrophoresis , homocysteine , methionine , chemistry , chromatography , biochemistry , amino acid
S ‐Adenosyl‐ L ‐homocysteine hydrolase (SAHh) inhibitors have long been used as broad‐range antivirals and have been recently evaluated as an experimental therapy of filovirus infections. In response to the need for a rapid laboratory testing method that could assess antiviral potency in vivo , our group developed a capillary electrophoresis (CE) method for the determination of the S ‐adenosyl‐ L ‐homocysteine (SAH) to S ‐adenosyl‐ L ‐methionine (SAM) ratio. After chloroacetaldehyde derivatization, SAH and SAM were detected using laser‐induced fluorescence detection with a HeCd laser. Separation and quantitation of both SAH and SAM in human plasma were achieved in less than 1 min. The proposed method is rapid and reliable, and could be easily applied to routine monitoring of clinical and preclinical trials subjects.