Premium
Injection by hydrostatic pressure in conjunction with electrokinetic force on a microfluidic chip
Author(s) -
Gai Hongwei,
Yu Linfen,
Dai Zhongpeng,
Ma Yinfa,
Lin Bingcheng
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305835
Subject(s) - electrokinetic phenomena , hydrostatic pressure , hydrostatic equilibrium , microfluidics , microfluidic chip , chromatography , fluorescein , fluorescein isothiocyanate , sodium fluorescein , chemistry , analytical chemistry (journal) , materials science , fluorescence , nanotechnology , mechanics , optics , physics , quantum mechanics
A simple method was developed for injecting a sample on a cross‐form microfluidic chip by means of hydrostatic pressure combined with electrokinetic forces. The hydrostatic pressure was generated simply by adjusting the liquid level in different reservoirs without any additional driven equipment such as a pump. Two dispensing strategies using a floating injection and a gated injection, coupled with hydrostatic pressure loading, were tested. The fluorescence observation verified the feasibility of hydrostatic pressure loading in the separation of a mixture of fluorescein sodium salt and fluorescein isothiocyanate. This method was proved to be effective in leading cells to a separation channel for single cell analysis.