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Determination of dissociation constants by competitive binding in partial filling capillary electrophoresis
Author(s) -
Nilsson Mikael,
Johansson Gunnar,
Isaksson Roland
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305786
Subject(s) - capillary electrophoresis , dissociation (chemistry) , dissociation constant , chemistry , dissociation rate , chromatography , electrophoresis , affinity electrophoresis , ligand (biochemistry) , competitive binding , capillary action , analytical chemistry (journal) , receptor , thermodynamics , biochemistry , affinity chromatography , enzyme , physics
The determination of dissociation constands ( K d ) by competitive ligand binding in partial filling capillary electrophoresis is demonstrated. Two different strategies were applied, one of which only uses a single reporter ligand and a more elaborated one which suppresses systemic disturbances by using a racemic mixture as reporter. The dissociation constants obtained by both alternatives were virtually identical and in good agreement with those previously reported.