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Optimization of sample stacking for the simultaneous determination of nonsteroidal anti‐inflammatory drugs with a wall‐coated histidine capillary column
Author(s) -
Pai YiFen,
Lin ChunChi,
Liu ChuenYing
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305780
Subject(s) - chemistry , chromatography , capillary electrochromatography , flurbiprofen , naproxen , detection limit , ketoprofen , ibuprofen , capillary electrophoresis , medicine , alternative medicine , pathology , pharmacology
A wall‐coated histidine capillary column was developed for the on‐line preconcentration of nonsteroidal anti‐inflammatory drugs (NSAIDs) in capillary electrochromatography (CEC). A wide variety of experimental parameters, such as the sample buffer, background electrolyte (BGE) composition, concentration, sample plug lengths, water plug, and the effect of organic modifiers were studied. The relationship between peak height and injection times for the NSAIDs by variation of sample and BGE buffer concentration was investigated. On addition of sodium chloride (0.3–0.6%) to the sample zone, the stacking efficiency was increased. With acetate buffer (100 m M , pH 5.0)/ethanol (20% v/v) as BGE and sample solution in acetate buffer (0.2 m M , pH 5.0)/ethanol (20% v/v)/NaCl (0.3% w/v), NSAIDs could be determined at low μ M levels without sample matrix removal. The detection limit was 0.096 μ M for indoprofen, 0.110 μ M for ketoprofen, 0.012 μ M for naproxen, 0.023 μ M for ibuprofen, 0.110 μ M for fenoprofen, 0.140 μ M for flurbiprofen, and 0.120 μ M for suprofen. The method could be successfully applied to the simultaneous determination of NSAIDs in urine. The recoveries were better than 82% for all the analytes. The present method enables simple manipulation with UV detection for the determination of NSAIDs at low concentration levels in complex matrix samples.