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Ultraviolet sealing and poly(dimethylacrylamide) modification for poly(dimethylsiloxane)/glass microchips
Author(s) -
Chen Lin,
Ren Jicun,
Bi Rui,
Chen Di
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305766
Subject(s) - contact angle , materials science , coating , capillary action , attenuated total reflection , analytical chemistry (journal) , infrared spectroscopy , reagent , ultraviolet , chemical engineering , chemistry , chromatography , nanotechnology , composite material , optoelectronics , organic chemistry , engineering
Simple sealing methods for poly(dimethylsiloxane) (PDMS)/glass‐based capillary electrophoresis (CE) microchips by UV irradiation are described. Further, we examined the possibility to modify the inner surface of separation channels, using polymethylacrylamide (PDMA) as a dynamic coating reagent. The surface properties of native PDMS, UV‐irradiated PDMS, and PDMA‐coated PDMS were systematically studied by atomic force microscopy (AFM), infrared absorption by attenuated total reflection infrared (ATR‐IR) spectroscopy, and contact angle measurement. We found that PDMA forms a stable coating on PDMS and glass surfaces, eliminating the nonhomogeneous electroosmotic flow (EOF) in channels on PDMS/glass microchips, and improving the hydrophilicity of PDMS surfaces. Mixtures of flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and fluorescein were separated in 35 s using PDMA‐coated PDMS/glass microchips. A high efficiency of theoretical plates with at least 1365 (105 000 N /m) and a good reproducibility with relative standard deviations (RSD) below 4% in five successive separations were achieved.