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Stir bar sorptive extraction‐liquid desorption applied to the analysis of hop‐derived bitter acids in beer by micellar electrokinetic chromatography
Author(s) -
De Villiers André,
Vanhoenacker Gerd,
Lynen Fréderic,
Sandra Pat
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305758
Subject(s) - chromatography , micellar electrokinetic chromatography , chemistry , electrokinetic phenomena , extraction (chemistry) , desorption , high performance liquid chromatography , sample preparation , detection limit , organic chemistry , adsorption
Stir bar sorptive extraction‐liquid desorption (SBSE‐LD) has been applied as an efficient sample preparation method for the analysis of beer bitter acids. Extracts free of almost all interfering compounds were obtained, allowing simultaneous analysis of iso‐α‐acids and reduced iso‐α‐acids. A robust micellar electrokinetic chromatography (MEKC) method was developed that enables fast separation of iso‐α‐acids and reduced iso‐α‐acids. Quantitative data are in good agreement with results obtained by high‐performance liquid chromatography (HPLC) using direct beer injection.