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High‐throughput single nucleotide polymorphism typing by fluorescent single‐strand conformation polymorphism analysis with capillary electrophoresis
Author(s) -
Doi Kent,
Doi Hitomi,
Noiri Eisei,
Nakao Akihide,
Fujita Toshiro,
Tokunaga Katsushi
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305721
Subject(s) - typing , capillary electrophoresis , single strand conformation polymorphism , single nucleotide polymorphism , snp , multiplex , multiplex polymerase chain reaction , microbiology and biotechnology , polymerase chain reaction , biology , genetics , dna , genotype , gene
In this study, we performed high‐throughput and precise single nucleotide polymorphism (SNP) typing by fluorescent capillary electrophoresis single‐strand conformation polymorphism (CE‐SSCP) analysis. A system composed of a multicapillary DNA analyzer, a newly developed sieving matrix, four different colors of fluorescent labels, and a multiplex polymerase chain reaction (PCR) enabled low‐cost and highly reliable SNP typing. Moreover, this system enabled the estimation of SNP allele frequencies using pooled DNA samples, which should be beneficial for large‐scale association studies. Thus, fluorescent CE‐SSCP analysis is a useful method for large‐scale SNP typing.