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Monomer surface modifications for rapid peptide analysis by capillary electrophoresis and capillary electrochromatography coupled to electrospray ionization‐mass spectrometry
Author(s) -
Johannesson Nina,
Wetterhall Magnus,
Markides Karin E.,
Bergquist Jonas
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305719
Subject(s) - capillary electrochromatography , chromatography , chemistry , capillary electrophoresis , electrochromatography , capillary electrophoresis–mass spectrometry , mass spectrometry , electrospray , capillary action , analytical chemistry (journal) , electrospray ionization , isoelectric focusing , electrophoresis , analyte , materials science , biochemistry , composite material , enzyme
Abstract In this study, positively charged alkylaminosilyl monomers were used to modify the inner surface of fused silica capillaries, which subsequently were employed in capillary electrophoresis (CE) and capillary electrochromatography (CEC). The obtained surfaces yield a reversed electroosmotic flow (EOF) and have varying carbon chain lengths, that interact with the analytes and give chromatographic retention. The coating procedure is very simple and fast. The performance of the modified capillaries was evaluated regarding pH influence on EOF and chromatographic interactions. The experiments were conducted with UV and mass spectrometry (MS) and applied to the separation of various neuropeptides. The derivatized surfaces showed a linear ( R 2 ∼0.99) pH dependence with isoelectric points (p I ) at 8.6–8.8. Rapid separations of peptide standards and a protein digest with efficiencies as high as 5×10 5 plates/m were performed.