Premium
Recent progress in high‐performance capillary bioseparations
Author(s) -
Huck Christian W.,
Stecher Günther,
Bakry Rania,
Bonn Günther K.
Publication year - 2003
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305717
Subject(s) - capillary electrophoresis , bioanalysis , analyte , proteome , chromatography , mass spectrometry , chemistry , biochemistry
Bioanalysis is a fast growing domain with new technological developments in the field of mass spectrometry, separation science, and bioinformatics. The central role of separation prior to detection and evaluation of analytes is indisputable. In fact, since the decipherment of the human genome via multichannel capillary electrophoresis in combination with laser‐induced fluorescence [1], the analysis of the proteome, i.e. , the type and amount of all proteins expressed at a certain time by the genome in a cell or tissue [2] became of central interest. Therefore, new fast and sensitive capillary liquid chromatographic, electrophoretic, and electrochromatographic separation techniques, suitable for high‐throughput analysis of proteins, peptides and nucleic acids, are required.