Premium
Open‐tubular capillary electrochromatography/electrospray ionization‐mass spectrometry using polymeric surfactant as a stationary phase coating
Author(s) -
Zhu Xiaofeng,
Kamande Mary W.,
Thiam Serigne,
Kapnissi Constantina P.,
Mwongela Simon M.,
Warner Isiah M.
Publication year - 2004
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.200305713
Subject(s) - capillary electrochromatography , mass spectrometry , chromatography , electrospray ionization , electrospray , pulmonary surfactant , electrochromatography , coating , capillary electrophoresis–mass spectrometry , chemistry , capillary action , stationary phase , analytical chemistry (journal) , materials science , organic chemistry , biochemistry , composite material
We describe the use of the polymeric surfactant poly(sodium undecylenic sulfate) (poly‐SUS) as a stationary phase coating in open‐tubular capillary electrochromatog raphy (OT‐CEC) coupled with electrospray ionization‐mass spectrometry (ESI‐MS) for the analysis of β‐blocker and benzodiazepine analytes. The production of a polymeric surfactant coating on the capillary inner wall involves (i) adsorption of the cationic polymer poly(diallyldimethylammonium chloride) (PDADMAC) to the inner surface of capillary, and (ii) adsorption of the negatively charged poly‐SUS onto the cationic polymer layer via strong physical interaction of the two polymer layers. As compared with micellar electrokinetic chromatography (MEKC) coupled with ESI‐MS, the main advantage of this proposed method is minimization of introduction of the monomeric or polymeric surfactant into the mass spectrometer, thus avoiding the interference of the nonvolatile micelle in ESI‐MS. The effects of buffer pH and applied voltage on the separation of the analytes are also discussed. Under optimum conditions, four of the five β‐blockers and four benzodiazepines are separated.